HSMB 561; HSMB 561C (CVP): Hematology (Lectures/Labs)
HSMB 520, HSMB 520C (CVP): Pathophysiology I
HSMB 612, HSMB 612C (CVP): Pathophysiology II
HSMB 687: Advanced Immunology
HSMB 256/HSMB656: Diagnostic Techniques and Molecular Pathology
HSMB 705: Selected Topics in Medical Biology:
Current concepts in Immunology.

HSMB 705 Leukocyte Interactions
HSMB 688: Immunochemistry (2004)


Hematology: HSMB 561/561C (FALL)

Molecular/Cell Biology; Introduction to hematology; Regulation of Erythropoisis;
Morphology of RBC, Hemoglobin; Iron, B12/folate metabolism;
Hemoglobin disorders; Heme synthesis disorders;
Introduction to anemia: Anemias, Microcytic, Macrocytic
Hemolytic diseases, Thalassemia, Hemoglobinopathy
Leukopoiesis; WBC morphology in peripheral blood and bone marrow;
Benign disorders of Leukopoiesis
Leukemias and lymphomas
Coagulation and coagulopathies.
Labs : Normal RBC morphology; RBC morphology in anemias; purification of cells, cell staining, Clinical cases
Normal WBC; WBC differentials; PCR, clinical cases.
Hematology instrumentation; research techniques; molecular diagnostic testing in
Hematology; distinguishing normal Vs abnormal RBC, WBC


Pathophysiology I - HSMB 520/520C (FALL)

Molecular/ Cell Biology of the Immune system
Inflammatory Response: Mediators of Inflammation
Pathophysiology of Type I to Type IV hypersensitive reactions
Autoimmune diseases
Congenital and Hereditary Diseases
Immune response to infectious diseases
Bacterial diseases
Viral diseases: Hepatitis;
Introduction to Hematology: Anemias
Leukemias and lymphomas; Coagulation - disorders
Cancer: classification, clinical features; Breast, colon, skin, prostrate cancers
Immune response to tumors and Cancer Immunotherapy
Clinical case discussions

Advanced Immunology: HSMB 687 (SPRING)
Immunoglobulins; Ig gene rearrangement;
Antibody engineering;
MHC class I and class II;
TCR, TCR-gene rearrangement; T cell signal transduction.
T cell maturation: positive and negative selection; T cell activation and differentiation.
Cytokines: inflammation, cytokine receptors; cytokine signaling, cytokine therapy.
Cell-mediated immune responses;
B cell activation; in vivo induction of humoral response; primary and secondary responses;
Immune deficiency diseases; Molecular Immunology of HIV.
Tumor immunology; immunotherapy. Transplantation immunology;
Apoptosis; Vaccines.

Pathophysiology II: HSMB 612/612C (SPRING)

Cardiovascular system:
Structure and function of the heart; cardiac muscle depolarization and action potential;
cardiac cycle; relationship between lungs and heart; nervous control of cardiac output;
blood pressure; hormonal control of the vascular system; congestive heart failure;
ischemic heart disease; angina pectoris; myocardial infarction; hypertensive heart disease;
cor pulmonale; valvular diseases; atherosclerosis.

Respiratory system:
Structure and function of the respiratory system; nervous control of respiration;
chemoreceptors; acid-base balance; upper respiratory diseases; COPD;
bronchiectasis; pneumothorax; pneumonia; asthma; ARDS; cystic fibrosis;
bronchogenic carcinoma.

Renal System:
Kidney: anatomy; functions of the kidney; renal function tests; renal absorption;
urinary tract infections; cystitis; pyelonephritis; glomerulonephritis; nephrotic syndrome; kidney stones; acute and chronic renal
failure; renal carcinoma.

Endocrine system:
Structure, function, and diseases of pituitary, thyroid, adrenal, parathyroid glands, and pancreas;
complications of diabetes.

Functions of the liver, jaundice, gall bladder, gallstones, pancreatitis.

Structure of the nervous system, degenerative diseases: MS, Parkinson’s disease, Huntington’s disease
Student presentations

Molecular diagnostics lab course: HSMB 656/256 (SPRING)

Introduction: Lab safety rules, video,
Introductory lectures on Cellular Immunology:
Preparation of cell culture medium; Cell preparation;
Cell counting, staining for surface markers,Cell culture techniques.
Cell proliferation assays: MTT, tritiated thymidine (3H-TdT) assays.
Monoclonal antibodies; CD numbers. Cell cloning; Flow cytometry
Some routine diagnostic tests used in the labs, Immunoassays:
Mixed lymphocyte reaction; CTL assay; Plaque assay, Allergic reactions.
Introduction to immunochemistry:
Polyacrylamide gel electrophoresis and Western blotting
Tyrosine phosphorylation studies: Cell stimulation; Cell lysis;
Immunoblotting, Immunoprecipitation, ELISA.
Introduction to molecular biology:
Restriction enzymes, Vectors, Cloning of cDNA and Genomic DNA, PCR,
RELP, Southern and Northern Blotting; DNA Finger Printing; DNA sequencing;
Gene Therapy.

Labs : Buffy coat preparation, staining with trypan blue to assess viability; purification
of T cells and staining T cells with anti-CD3-FITC antibody to check surface staining.

Affinity purification;
Gel electrophoresis, Western blotting,
Quantitative ELISA

Purification of Chromosomal DNA and Plasmid DNA
Restriction analysis;
DNA finger printing I- identification of DNA by restriction fragmentation patterns
Transformation of E.coli with pGAL blue colony

Leukocyte Interactions (HSMB 705, Spring)

Introduction: Organs and Cells of the Immune System. Cell surface molecules
Antigen processing and presentation,
Hybridoma generation and Monoclonal antibodies;
Development of T cells; positive and negative selection
Role of co-receptors, T-B activation
Experimental systems
Humoral/ cell-mediated immune responses

Journal article presentation by the students:

Journals: Journal of Immunology; Cellular Immunology;
Proc. Natl.Acad. Science; Science; Nature (London);
European Journal of Immunology;
Journal of Experimental Medicine;
Blood; Nature Medicine; Immunity;
AIDS; Clinical Immunology and any other immunology/molecular immunology journals..

Immunochemistry HSMB 688 (2004, Spring) (Lectures and Labs)

This course illustrates the quantitative and qualitative aspects of immunochemistry. Ouchterlony; Radial Immunodiffusion; Thin layer Chromatography; Immuno-electrophoresis;
Separation of RNA and DNA by gel filtration chromatography
Analysis of native proteins by Electrophoresis; ELISA; Surface Staining of cells.
Dot Blot Assay; Western Blot; Quantitative ELISA, FACS

Current Concepts in Immunology HSMB 705: (Summer I)
Over view of Immunology
Important techniques in molecular Immunology
Discussion on current research areas
Discussion on how to review papers, how to write thesis, an introduction and discussion on all the topics to be discussed by the students.
student presentations: clinical cases/journal articles:

HSMB 706 Library Research
HSMB707/708 Experimental Research